bmpr2 coding sequence Search Results


bmpr2 coding sequence  (Addgene inc)
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Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the <t>BMPR1A-BMPR2</t> complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.
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Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.

Journal: Gastroenterology

Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

doi: 10.1053/j.gastro.2023.03.006

Figure Lengend Snippet: Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.

Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

Techniques: Phospho-proteomics

Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

Journal: Gastroenterology

Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

doi: 10.1053/j.gastro.2023.03.006

Figure Lengend Snippet: Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

Techniques: Inhibition, Clone Assay, Proliferation Assay, Colony Assay

Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

Journal: Gastroenterology

Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

doi: 10.1053/j.gastro.2023.03.006

Figure Lengend Snippet: Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

Techniques: Activity Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing